International journal of nanomedicine
Sequential Barrier Navigation by Yeast β-Glucan-Engineered Liposomal Nanovaccines Potentiates Oral Melanoma Immunotherapy
By Wu D, Jiang C, Li H and 10 others.
Abstract
Purpose
Orally delivered protein antigens face gastrointestinal degradation and inefficient transport through mucus, across the intestinal epithelium, and into antigen-presenting cells (APCs). To address these barriers, we developed YGB@OVA/PBA-C-LIPs, an ovalbumin (OVA)-loaded cationic liposomal nanovaccine coated with yeast β-glucan (YGB) and functionalized with phenylboronic acid (PBA).
Methods
We characterized the physicochemical properties of YGB@OVA/PBA-C-LIPs and evaluated their mucus and epithelial transport, macrophage uptake, OVA cross-presentation, and immune activation in vitro. Laminarin competition was used to assess β-glucan-sensitive uptake. Gastrointestinal residence, immune responses, antitumor activity, and tolerability were examined after oral administration to B16-OVA-bearing mice.