Cells
Inhibition of FAK-Actin Pathway by Indoxyl Sulfate Suppresses Microvascular Remodeling in a Mouse Model of Chronic Kidney Disease
By Sean Carr, Tharmarajan Ramprasath and Ming‐Hui Zou.
Abstract
Objective
Chronic kidney disease (CKD) worsens outcomes of peripheral artery diseases (PADs), partly through impaired vascular regeneration and microvascular remodeling. CKD promotes the accumulation of uremic toxins, but their contributions to microvascular dysfunction remain poorly understood. This study investigated the effects of uremic toxin indoxyl sulfate (IS) on vascular smooth muscle cell (VSMC) function and microvascular remodeling in a mouse model of CKD.
Methods
C57BL6/J mice were fed 0.25% adenine diet for 10 days to induce CKD. CKD mice underwent hindlimb ligation surgery. VSMC migration assays, microscopy, and Western blotting were utilized to elucidate the underlying mechanisms underlying the IS-induced impairment of vascular remodeling.