Cancers
YY1 Suppresses Tumor Growth by Negatively Regulating the Expression and Translation Activity of eIF4E in Nasopharyngeal Carcinoma
By Yumei Duan, Xiangting Zhou, Jianxia Wei and 9 others.
Abstract
Background
Nasopharyngeal carcinoma (NPC) is a complex malignancy, and the molecular mechanisms that drive its progression have not yet been fully elucidated. Eukaryotic translation initiation factor 4E (eIF4E), a pivotal regulator of protein synthesis, is frequently dysregulated in cancers. We previously identified eIF4E as a direct transcriptional target of Yin Yang 1 (YY1) in an axis where YY1 suppressed NPC invasion and metastasis.
Methods
Here, we investigated the function of eIF4E in NPC growth and its regulatory relationship with YY1. The CCK-8 assay, clonogenic survival assay, Annexin V/propidium iodide assay, and xenograft models were used to determine the role of YY1/eIF4E in NPC growth; Western blotting and Co-IP were performed to examine protein expression and protein–protein interactions, respectively.