Pharmaceutics
Multifunctional Hyaluronic Acid-Modified Pterostilbene Nanoliposomes for Enhanced Dermal Delivery and Extracellular Matrix Protection
By Wang Z, Fan Y and Ling P.
Abstract
Background
Skin aging involves extracellular matrix (ECM) deterioration, including collagen loss and disrupted glycosaminoglycan and proteoglycan homeostasis. Pterostilbene (PTE) has antioxidant and anti-inflammatory activity, but its topical use is limited by poor aqueous solubility and limited skin delivery. We developed hyaluronic acid (HA)-modified, PTE-loaded nanoliposomes (HA-LP-PTE) and evaluated their association with dermal fibroblasts, retention in skin, and effects on ECM-related responses.
Methods
HA-LP-PTE was prepared by high-pressure microfluidization and assessed for size, morphology, zeta potential, and encapsulation efficiency. Fluorescence microscopy was used to examine fibroblast-associated fluorescence of FITC-labeled HA-LP-PTE and non-HA-modified PTE-loaded liposomes (LP-PTE). Skin retention was assessed using Franz diffusion cells. ECM-related changes were analyzed in UV-irradiated ex vivo skin and fibroblasts using staining techniques and RT-qPCR.