Life
Preoperative Assessment of Circulating Extracellular Vesicles as a Potential Biomarker for Melanoma
By Gualdi G, Amerio P, Marani A and 10 others.
Abstract
Background
Circulating extracellular vesicle (EV)-associated particle profiles may differ between patients with melanoma and those with benign lesions.
Methods
Preoperative citrated whole blood from 77 patients with clinically suspicious lesions was assessed by direct fluorescence-based flow cytometry; histopathology identified 20 CMM cases (12 primary and 8 metastatic) and 57 benign-lesion controls.
Results
The reported total particle concentration was higher in CMM than controls (3299.86 ± 456.61 vs. 2873.40 ± 427.74 events/µL). CD45+, PD-L1+, and CD45+/PD-L1+ populations were also increased. CD45+/VISTA+ particles were higher in the reduced subset of 9 CMM cases and 48 controls.