Biomedicines
Phenotypic Landscape of Programmed Death and TIGIT/CD155/DNAM-1 Pathways in NK Cells in Ovarian Cancer
By Pawłowska-Łachut A, Skiba W, Pieniądz-Feculak P and 9 others.
Abstract
Objectives
Ovarian cancer (OC) is the gynecological malignancy with the highest mortality rate. Preclinical studies have shown that dual blockade of immune checkpoint proteins (ICPs), such as PD-1 and TIGIT, may be beneficial for OC patients. Thus, the aim of this study was to evaluate the expression of PD-1, TIGIT, and DNAM-1 in NK cells in three OC environments-i.e., peripheral blood (PB), peritoneal fluid (PF), and tumor tissue (TT)-and their soluble forms in plasma and PF to explore their clinical significance.
Methods
The expression of ICPs on NK cells was analyzed via flow cytometry. The concentrations of soluble forms of these ICPs were measured in plasma and PF via ELISA. The obtained results were correlated with the clinicopathological features of OC patients.