Genes
MiR-4261 Regulates Granulosa Cell Proliferation Mediated by Progesterone via FZD5 in Human Ovarian Granulosa Cell Line
By Yu J, Zhang Y, Kuai Y and 2 others.
Abstract
Background
The effect of different controlled ovarian hyperstimulation (COH) protocols on the microenvironment of follicular growth may be reflected by microRNA (miRNA) expression profiles. Downregulation of miR-4261 when progesterone is added in the follicular phase has been reported in our previous research. The purpose of this study is to observe how miR-4261 regulates the function of granulosa cells, so as to find the specific pathway and mechanism of progesterone acting on the follicular microenvironment.
Results
The human ovarian granulosa cell line (KGN cells) is used to conduct cell experiments as granulosa cells. Compared with microRNA-negative control (miR-NC), KGN cells transfected with the miR-4261 mimic showed progressively reduced proliferation at 24, 48, and 72 h (p < 0.01). The levels of Frizzled 5 (FZD5) mRNA and protein were also significantly reduced (p < 0.01). In the dual-luciferase reporter gene experiment, the fluorescence value of KGN cells co-transfected with wild type-FZD5 (wt-FZD5) and the miR-4261 mimic/inhibitor decreased/increased (p < 0.01), and there was no significant difference in fluorescence value between mutant-FZD5 (mut-FZD5) and the miR-4261 mimic/inhibitor co-transfected KGN cells (p > 0.05). The proliferation activity of KGN cells transfected with small interfering FZD5 (siFZD5) decreased significantly at 48 h (p < 0.01) compared with the control. Compared with KGN cells co-transfected with siFZD5 and miR-NC, there was no significant difference in the proliferation activity of KGN cells co-transfected with siFZD5 and miR-4261 inhibitor (p > 0.05).