Journal of inflammation research
Integrative Analysis of m6A Methylation and Transcriptomic Profiles in Cortical Tissues of Mice with Sepsis-Associated Encephalopathy
By Zhao J, Zhu T, Zhao C and 6 others.
Abstract
Background
Sepsis-associated encephalopathy (SAE) is a common and severe complication in septic patients, characterized by the increased mortality and cognition dysfunction. N6-Methyladenosine (m6A), one of the most prevalent RNA modifications, plays crucial roles in the central nervous system by regulating RNA metabolism. However, its involvement in the pathogenesis of SAE remains unexplored.
Methods
RNA sequencing (RNA-seq) and methylated RNA immunoprecipitation (MeRIP)-sequencing were performed to analyze the transcriptomic profiles and m6A methylation in the brain cortex of LPS-induced septic mice. Bioinformatics analysis was carried out to identify genes with altered m6A methylation and differential expression. Target genes and m6A regulators were further validated using quantitative reverse transcription polymerase chain reaction (RT-qPCR).