Membranes
Bilayer Polycaprolactone (Mitomycin C)/Poly(L-Lactic Acid)/Gelatine (Dimethyloxalylglycine) Nanofibres for Diabetic Wound Repair
By Zhu R, Wang Q, Chen Z and 9 others.
Abstract
Background
Diabetic skin wounds arise from chronic hyperglycaemia, peripheral neuropathy, microvascular dysfunction, and compromised immunity. Chronic ulcers are further complicated by infection and local ischaemia-hypoxia, which collectively impede tissue repair. Conventional interventions, including glycaemic control, anti-infective therapy, debridement, and wound dressings, can only partially relieve clinical symptoms. Since these strategies fail to reverse ischaemia-hypoxia or restore the reparative capacity of endogenous cells, ulcers often recur, healing outcomes remain unsatisfactory, and limb amputation may become unavoidable. To overcome these drawbacks, we fabricated a bilayer electrospun microfibre membrane for diabetic wound repair.
Methods
An electrospun microfibre membrane with a bilayer structure was fabricated. The outer layer, composed of polycaprolactone (PCL), provided mechanical support and enabled sustained release of mitomycin C (MMC). The inner layer, formulated with a 7:3 ratio of poly-L-lactic acid (PLLA) to gelatine (Gel), was designed to match the mechanical properties of the wound bed and was loaded with dimethyloxalylglycine (DMOG). Antibacterial assays against Escherichia coli and Staphylococcus aureus, as well as proangiogenic evaluations in ischaemic tissues, were conducted to characterise this composite membrane.