Dentistry journal
Xenogeneic Grafts Combined with Platelet Concentrates: Effects on Bone Microarchitecture and Collagen Maturation
By Braga WTS, Martins UDCC, Sávio DSF and 6 others.
Abstract
Background
This study assessed the impact of xenogeneic bone grafts (XEN), either utilized alone or combined with autologous platelet concentrates (APCs) prepared using three different centrifugation protocols, on the healing of critical-size defects (CSDs) in rat calvaria.
Methods
Forty rats were assigned to five groups (n = 8): Control, XEN, XEN + L-PRF, XEN + A-PRF, and XEN + Bio-PRF. Blood concentrates were prepared by centrifuging blood collected via cardiac puncture: L-PRF (~700 g for 12 min), A-PRF (~208 g for 14 min), and Bio-PRF (~700 g for 8 min). Defects (5 mm diameter) in the right parietal bone of each rat were filled with blood clot, XEN, or XEN combined with L-PRF, A-PRF, or Bio-PRF. Animals were euthanized 60 days post-surgery, and the calvariae were analyzed using histomorphometry and micro-CT. The percentage of mature and immature collagen fibers was analyzed using Picrosirius red staining under polarized light microscopy.