Iranian journal of biotechnology
Differential Regulation of Postoperative Gut Microbiota by Inhalation vs Intravenous Anesthesia: NF-κB/HIF-1α-Mediated Macrophage Polarization
By Chen N, Hu C, Huang X and 1 others.
Abstract
Background
Perioperative anesthetic strategies are increasingly recognized as important modulators of postoperative immune responses and intestinal microecological homeostasis following gastrointestinal surgery.
Objective
To systematically explore the differential effects of inhalational anesthesia (sevoflurane) and intravenous anesthesia (propofol) on postoperative intestinal microbiota, and to investigate the potential association between anesthetic methods, NF-κB/HIF-1α signaling pathway, and macrophage polarization using integrated bioinformatics analyses.
Methods
Publicly available datasets were retrieved from NCBI SRA, GEO, and EMBL-EBI to identify three independent cohorts with clear annotations of gastrointestinal surgery and anesthetic methods. A total of 90 samples were included (30 control, 30 inhalational anesthesia, and 30 intravenous anesthesia). Intestinal microbiota profiles derived from 16S rRNA sequencing were integrated with expression data of NF-κB/HIF-1α pathway-related genes. Microbial diversity, community composition, differential taxa, functional enrichment, and microbe-gene correlations were analyzed using QIIME2, limma, vegan, and related bioinformatics tools to construct the regulatory axis of "anesthetic method-intestinal microbiota-signaling pathway-macrophage polarization."