International journal of women's health
Single-Cell Transcriptomics Reveals Rora-Associated Aberrant Granulosa Cell Differentiation in Polycystic Ovary Syndrome
By Ji P, Sun S, Xu X and 1 others.
Abstract
Introduction
Polycystic ovary syndrome (PCOS) is characterized by follicular developmental arrest, yet the molecular basis underlying impaired granulosa cell (GC) differentiation remains incompletely understood.
Methods
We constructed a single-cell transcriptomic atlas of ovaries from DHEA-induced PCOS-like mice and matched vehicle controls, and leveraged spatial transcriptomics as an orthogonal reference for major cell-type annotation, followed by in-depth profiling of GC heterogeneity. Pseudotime inference, co-expression networks, and regulon activity analysis were integrated, and findings were validated in independent human GC datasets. In silico Rora perturbation, cell-cell communication analysis, and drug repositioning were additionally performed.