Journal of dental sciences
Developing an automated cytology-based DNA ploidy test for detecting oral squamous cell carcinoma in Taiwan: A feasibility study
By Lu PH, Liu KYP, Chang JYF and 5 others.
Abstract
Background/purpose
Oral squamous cell carcinoma (OSCC) remains a global health issue, particularly in underserved settings such Taiwan where access to early diagnosis is limited. DNA ploidy-based oral cytology has shown strong diagnostic performance, but its applicability across populations require evaluation.
Materials and methods
In this prospective feasibility study, brush and oral rinse samples were collected from patients with OSCC, low-grade dysplasia, reactive lesions, and normal mucosa. DNA cytometry was performed to quantify ploidy profiles. Diagnostic performance was evaluated using published algorithm and cohort-specific thresholds derived from stratified 10-fold cross-validation.