Frontiers in cell and developmental biology
Potential anti-osteoporotic and anti-inflammatory potential of plant derived extracts and column semi-purified fractions of Epimedium brevicornum
By Ding N and Wang B.
Abstract
Background
Osteoporosis is characterized by excessive bone resorption and impaired bone remodeling, often accompanied by inflammatory processes that exacerbate skeletal deterioration. Natural products with multi-target potential have gained attention as alternative therapeutic strategies. The present study aimed to investigate the in-vitro anti-osteoporotic and anti-inflammatory potential of extracts obtained from different parts of Epimedium brevicornum, including leaves, stem, and roots, as well as isolated column semi-purified fractions (E-B-1 C, E-B-2 C, E-B-3 C, and E-B-4 C) derived from the leaf extract.
Methods
Crude methanolic extracts and leaf-derived semi-purified fractions were prepared using column chromatography and evaluated for inhibitory activity against key enzymes involved in bone resorption and remodeling, including tartrate-resistant acid phosphatase (TRAP), cathepsin K, matrix metalloproteinase-9 (MMP-9), and matrix metalloproteinase-13 (MMP-13). Anti-inflammatory activity was assessed through inhibition of cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX). Enzyme inhibition assays were conducted at varying concentrations to determine percentage inhibition and IC50.