Analytical cellular pathology
AC104024.2/miR-30a-5p Axis Regulates Malignant Progression of Breast Cancer Through a Depression-Related Gene, MAP2K3
By Li J, Sun J, Jiang L and 3 others.
Abstract
Background
Breast cancer (BC), a malignant tumor, is characterized by high incidence and poor prognosis among women worldwide. Long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) can regulate the initiation and progression of BC through competitive endogenous RNA (ceRNA) mechanisms. Despite depression being an important factor affecting patients with BC, the ceRNA regulatory network involving depression-related genes in BC remains unclear.
Methods
In this study, we explored the role of the AC104024.2/miR-30a-5p axis in BC progression by targeting the depression-related gene mitogen-activated protein kinase kinase 3 (MAP2K3). We used depression-like mouse models to confirm the role of MAP2K3 in modulating depressive behavior. Additionally, gene set enrichment analyses (GSEA) and phenotypic experiments were conducted to verify the effects of the AC104024.2/miR-30a-5p/MAP2K3 axis on BC cell proliferation, migration, and apoptosis. Luciferase reporter assays were performed to demonstrate the ceRNA function of AC104024.2 for miR-30a-5p.