Avicenna journal of phytomedicine
Dorema aucheri extract for reducing oxidative stress and kidney injuries in diabetic rats
By Raeisi A, Koohpeyma F, Siri M and 6 others.
Abstract
Objective
Diabetic hyperglycemia causes oxidative stress, contributing to chronic kidney disease (CKD). This study evaluated the effects of ethanolic Dorema aucheri extract from aerial parts on oxidative stress, inflammation, and kidney injury in diabetic rats.
Materials and methods
Thirty male rats were randomly assigned to five groups (n=6): non-diabetic control, diabetic control, diabetic + metformin (500 mg/kg/day), and diabetic + D. aucheri extract (250 or 500 mg/kg/day). Treatments were given orally for 28 days. Serum and kidney samples were analyzed for fasting blood glucose (FBS), renal function [blood urea nitrogen (BUN), creatinine (Cr)], inflammatory cytokines [interleukin-6 (IL-6), interleukin-1β (IL-1β)], oxidative stress [malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx)], mRNA expression [kidney injury molecule-1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL), B-cell lymphoma 2 (BCL-2), Caspase-9 (CASP-9)], stereology, and histopathology.