Frontiers in pharmacology
Phytochemical characterization and effects of Dolomiaea costus root extract on ovariectomy-induced metabolic, skeletal, and uterine alterations in rats
By Aldayel TS, Mahmoud HS, Abdelrazek HMA and 4 others.
Abstract
Background
Estrogen deficiency following ovariectomy produces metabolic, skeletal, and uterine alterations.
Methods
In this study, we evaluated an authenticated and chemically characterized dichloromethane-methanol (1:1, v/v) root extract of Dolomiaea costus (Syn. Saussurea costus) in ovariectomized female Wistar rats. Thirty rats were assigned to sham-operated, ovariectomized untreated, and ovariectomized extract-treated groups. Beginning 3 weeks after surgery, the treated group received the extract orally at 300 mg/kg body weight/day for 12 weeks. Metabolic and lipid variables, adipokines, antioxidant status, and bone-related biomarkers were determined. Uterine, femoral, hepatic, and adipose tissues were examined histologically, while estrogen receptor-α (ER-α) and tumor necrosis factor-α (TNF- α) immunoreactivity were evaluated. The extract was characterized using spectrophotometric assays, GC-MS, and HPLC-DAD.