PloS one
Vimentin neutralizing antibody ameliorate inflammatory bowel disease by inhibiting fibrosis and inflammatory cell death
By Kim S, Kang HY, Park JS and 6 others.
Abstract
Background
Inflammatory bowel disease (IBD) is characterized by chronic inflammation intertwined with fibrosis and increased necroptosis. Vimentin, an intermediate filament protein closely associated with fibrosis and several inflammatory pathways, is elevated in IBD tissues. We conducted experiments to determine the effect of neutralization of extracellular vimentin with an anti-vimentin antibody (Ab) on the pathogenesis of IBD.
Methods
IBD was mimicked in a 7-week C57BL6 mice by mixing 3% dextran sodium sulfate (DSS) in autoclaved drinking water for five days. Anti-vimentin Ab was intraperitoneally injected with a concentration of 3 milligrams per kilogram repeatedly in a three-day interval simultaneously with DSS administration.