Frontiers in cellular and infection microbiology
Gut microbiota and serum metabolomics in children with human parainfluenza virus infection
By Ren X, Liu Z, Huang L and 9 others.
Abstract
Objective
Based on gut microbiota and serum untargeted metabolomics analyses, this study investigated changes in gut microbiota structure and serum metabolic profiles in children infected with human parainfluenza virus (HPIV). By integrating multi-omics data, we aimed to explore interactions between gut dysbiosis and serum metabolic disorders, and their potential roles in HPIV pathogenesis, to provide preliminary evidence for microbiota--targeted and metabolic intervention strategies.
Methods
Clinical data and laboratory results were collected from 230 hospitalized children (<14 years) with HPIV infection. Subsequently, 31 HPIV-infected children and 12 healthy controls were enrolled. Fecal samples were analyzed via 16S rRNA gene sequencing, and serum samples via liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based untargeted metabolomics, followed by integrated multi-omics analysis.