Frontiers in immunology
Identification of heterogeneous subtypes of human dendritic cells from the lymph nodes of patients with gastric cancer
By Han SH, Lee IJ, Tran HVA and 7 others.
Abstract
Objective
The subtypes of heterogeneous conventional dendritic cells (cDCs) and plasmacytoid dendritic cells (pDCs) in lymph nodes (LN) cannot be distinguished because they share similar surface markers. This study aimed to identify cDCs, pDCs, and other cell types in DC-enriched LNs from patients with gastric cancer (GC) by analyzing surface markers and gene expression.
Methods
Expression of surface markers, including CD141, CD1c, and CD123, in DC-enriched cells was analyzed by conventional flow cytometry and AbSeq using antibody-derived tags. Transcriptome and epitope analyses were used to characterize DC subtypes. The frequencies of heterogeneous DC subtypes were compared across clinical stages and molecular subtypes in patients with GC.