International journal of genomics
Targeting lncRNA PVT1/miR-145-5p/ABCC-1 Pathway Enhances Paclitaxel Sensitivity and Suppresses Melanoma Progression
By Pedram N, Safaralizadeh R, Feizi MAH and 1 others.
Abstract
Background
As a highly aggressive type of skin cancer, melanoma cells show significant resistance against a variety of chemotherapeutics, such as paclitaxel, which limits the efficacy of the treatment. Studies have highlighted the potential role of noncoding RNA dysregulation in melanoma, affecting tumor progression and drug response, including lncRNA PVT1.
Purpose
This study investigated whether simultaneous modulation of the lncRNA PVT1/miR-145-5p/ABCC1 axis enhances paclitaxel sensitivity and suppresses malignant behaviors in a melanoma cell line.
Methods
Using SK-MEL-3 and A375 cells, we performed PVT1 knockdown using siPVT1, restored miR-145-5p function by transfection, and administered paclitaxel, either alone or in combination. Cell viability was assessed using the MTT assay; apoptosis and cell cycle distribution were analyzed by flow cytometry; and the expression of genes related to apoptosis, cell cycle regulation, migration, and drug resistance was quantified by qRT-PCR.