Integrative characterization of human hepatocellular carcinoma-derived organoids and their HBV-associated biology

Background

Hepatocellular carcinoma (HCC) is one of the most aggressive malignancies and is characterized by a high degree of heterogeneity. However, HCC treatment remains difficult, highlighting the need to better define molecular subgroups of HCC and understand their potential relationships with therapeutic response.

Methods

In this study, we established 20 patient-derived organoids and 12 patient-derived cell lines from 20 individuals diagnosed with HCC. These models were comprehensively characterized using whole-exome sequencing, RNA sequencing, drug screening, and hepatitis B virus (HBV)-targeting PCR with a focus on HBV-related HCC.

Results