International journal of nanomedicine
A Preliminary in-vitro Evaluation of Serum Capped Colloidal Silver Nanoparticles on Liver Cancer towards Development of Safe Personalized Nanomedicine
By Parveen R, Maiti PK, Arab MM and 3 others.
Abstract
Background
Serum-capped silver nanoparticles (AgNPs), synthesized using naturally occurring serum proteins and reducing agents, represent a green nanotechnology platform with potential for safer and more selective therapy. This study compared the safety and anticancer efficacy of serum-capped AgNPs with polyvinyl alcohol (PVA)-capped AgNPs.
Methods
Serum-capped AgNPs (~18 nm, zeta potential -8.9 mV) were synthesized from human serum and administered intravenously in mice (0.1 mL/dose). PVA-capped AgNPs of equivalent silver concentration served as controls. Effects were assessed in a mouse hepatoma model, WRL-68, and Hep-G2, two different liver-derived cell lines using histopathology, biochemical analysis, MTT assay, estimation of proliferative potential by cell cluster formation assay, scratch assay, and hexosamine assays.