Iranian journal of basic medical sciences
Protein kinase C θ deletion ameliorates bleomycin-induced pulmonary fibrosis via the improvement of SIRT1/AMPK signaling on autophagy and senescence
By Li W, Zhao X, Wang M and 3 others.
Abstract
Objectives
Protein kinase C theta (PKC θ) is involved in the regulation of various inflammatory diseases, such as allergic asthma and ConA-induced hepatitis. However, its role in pulmonary fibrosis has not been reported. This study aimed to explore the role of PKC θ in bleomycin-induced pulmonary fibrosis and the potential mechanisms involved.
Materials and methods
WT C57BL/6 and PKC-θ deletion mice were administered a single intratracheal injection of bleomycin at a dosage of 3 mg/kg to prepare an animal model of pulmonary fibrosis. Histopathological changes of lung fibrosis were observed. Blood samples and BALF were collected for biochemical analysis. Protein expression in lung tissues was assessed to investigate a potential mechanism of PKC θ in pulmonary fibrosis.