Open Journal of Physicians and Surgeons
Hyperglycemia-Induced miR-155 Amplifies Inflammatory Cytokine Secretion in Human Mesangial Cells via SOCS1 Suppression
By Narasimha Sridevi Anand, Kamshid L. Gupta, Shanthi N. Korrapati.
Abstract
Background
Diabetic nephropathy is characterized by chronic low-grade inflammation within renal tissues. MicroRNA-155 (miR-155) is a key regulator of inflammatory cascades; however, its role in mesangial cell injury under hyperglycemic conditions requires clarification.
Objectives
To evaluate miR-155 expression and its effect on pro-inflammatory cytokine secretion in cultured human mesangial cells exposed to high glucose concentrations.
Materials and methods
Primary human mesangial cells were cultured under normal (5.5 mM) or high (25 mM) D-glucose conditions. Expression of miR-155 was quantified using quantitative real-time RT-PCR. Cells were transfected with miR-155 mimics or inhibitors, and secretion of IL-6, TNF-α, and MCP-1 was measured by ELISA.