Hammurabi Journal of Medical Sciences
Association of Salivary Pepsin with Systemic Inflammatory Biomarkers and Laryngeal Disease Severity in Patients with Laryngopharyngeal Reflux–Associated Chronic Laryngitis
By Muthanna Saleem Abdulameer.
Abstract
Background
Laryngopharyngeal reflux (LPR) is clinically heterogeneous and symptoms and/or laryngoscopic signs alone have limited specificity. While salivary pepsin is a non-invasive measure of gastric-content exposure, its association with systemic inflammation and severity of laryngeal disease is not well described.
Objectives
to assess the correlation between salivary pepsin concentration and the systemic inflammatory biomarkers in chronic laryngitis associated with LPR. Materials and Methods: This was a prospective analytical comparative case-control study involving 180 adults: 60 patients with LPR-associated chronic laryngitis, 60 patients with chronic laryngitis without objective evidence of LPR and 60 healthy controls. Three timed saliva samples were analysed for pepsin A using quantitative enzyme-linked immunosorbent assay (ELISA). High sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), complete blood count-derived indices, Reflux Symptom Score-12 (RSS-12) and Reflux Sign Assessment-10 (RSA-10) were assessed. Multivariable models and receiver operating characteristic (ROC) analyses were used.