Pharmaceuticals
Functionalization of Metal-Enriched Pleurotus Biomass with γ-Poly-Glutamic Acid for Cytoprotective and Antimicrobial Wound Care
By Agata Krakowska, Alicja Matyjewicz, Iwona Skiba‐Kurek and 6 others.
Abstract
Objectives
Chronic wound dressings require active components that support moisture management, biocompatibility, and local bioburden control. Therefore, in this study, freeze-dried biomass of Pleurotus ostreatus (Po) and Pleurotus djamor (Pdj) was cultivated in vitro under mineral-enriched conditions (calcium, magnesium, zinc) and subsequently modified with poly(γ-glutamic acid) (γ-PGA) to develop a bioactive fungal matrix intended as a functional additive for future wound-care biomaterials.
Methods
The elemental content of the biomass was determined using atomic absorption spectroscopy (AAS), and the resulting biomaterial underwent physicochemical analyses—including scanning electron microscopy, X-ray diffraction (XRD), wettability measurements, differential scanning calorimetry (DSC), and thermogravimetric analysis (TG)—as well as biological performance assessments using a disk diffusion assay and cytotoxicity analysis.