Pharmaceuticals
Interventional Effects and Neurometabolic Regulation of a Panax quinquefolius-Containing Herbal Extract (JYF) in Diazepam Withdrawal Syndrome in Mice
By Yue Jiao, Chang Gao, Peng Li and 6 others.
Abstract
Background
Long-term benzodiazepine use can lead to dependence and clinically significant withdrawal following discontinuation, highlighting the need to identify effective interventions for withdrawal-associated abnormalities.
Methods
High-resolution mass spectrometry was used to characterize the constituents of a Panax quinquefolius-containing herbal extract (JYF) and evaluate their in vivo exposure in rats. Sixty-five C57BL/6 mice were randomly assigned to control, model, and low-, medium-, and high-dose JYF groups. Diazepam withdrawal was induced after 28 days of subcutaneous diazepam administration (12 mg/kg), and JYF was administered orally during the peri-withdrawal period for 7 days, beginning 4 days before diazepam discontinuation. Behavioral assessments, electroencephalographic/electromyographic (EEG/EMG) recordings, histopathological analysis, and neurochemical measurements were performed. Network proximity analysis, widely targeted metabolomics, targeted neurotransmitter quantification, and integrated pathway analysis were subsequently performed.