Medical Sciences
Fluorescence Profiling of Urine in Non-Invasive Diagnostics of Obstructive and Reflux Uropathies in Children
By Anastasiya A. Luenkova, Галина Игоревна Кузовлева, Marina A. Korshunova and 6 others.
Abstract
Background
Rapid screening methods reflecting the metabolic profile of urine are promising for assessing the risk of decreased renal function in children with obstructive uropathies. The aim of this pilot study was to evaluate the applicability of a reaction-based fingerprinting strategy for this purpose, which has previously demonstrated its effectiveness in recognizing samples with similar composition.
Methods
The study included 28 children with hydronephrosis (HN, 5 patients) and vesicoureteral reflux (VUR, 15 patients), as well as healthy children without urinary pathology who comprised the control group (8 patients). Using the kinetic variant of optical fingerprinting, six indicator reactions of different natures involving dyes were investigated, with absorbance and fluorescence of the reaction products monitored photographically throughout the reaction.