Genes
Multi-Cohort Transcriptomic Integration Identifies Overlapping Immune and Antigen-Presentation Features in Alzheimer’s Disease and Hashimoto’s Thyroiditis
By Shuting Zhong, Guanfeng Xie and Zhaohui Zhang.
Abstract
Background
Thyroid disorders and Alzheimer’s disease (AD) have been linked epidemiologically, but whether AD brain tissue and thyroid tissue affected by Hashimoto’s thyroiditis (HT) share molecular features remains unclear.
Methods
Differential expression was estimated in AD (GSE122063) and HT (GSE138198) discovery cohorts using limma, with donor-level blocking and adjustment for age, sex and post-mortem interval where available. Shared genes were tested against chance within the genes measured in both cohorts, separately for each direction of change; characterized by enrichment analysis; related to marker-based cell-state scores; and evaluated in two AD cohorts, three thyroid cohorts including one with concurrently collected normal thyroid (GSE165724), and a single-nucleus AD dataset (GSE138852).