Investigative Ophthalmology & Visual Science
Neuroprotective and Anti-Inflammatory Effects of SOCS1 Mimetic Peptides in Mouse Diabetic Retinopathy
By Elena Salobrar‐García, Lucas Opazo-Ríos, José A. Matamoros and 10 others.
Abstract
Purpose
To evaluate the therapeutic potential of a suppressor of cytokine signaling 1 (SOCS1) mimetic peptide (MiS1) via systemic administration in a mouse model of diabetic retinopathy (DR).
Methods
Male db/db mice, a model of type 2 diabetes, were treated for 7 weeks with intraperitoneal injections of MiS1. In comparison, a group of animals received the Janus kinase (JAK)1/2 inhibitor baricitinib. Untreated diabetic db/db mice and non-diabetic db/m littermates served as controls. Retinal structure was assessed in vivo by optical coherence tomography. Retinal ganglion cell (RGC) survival (Brn3a+ cells) and Iba1-positive microglial activation (Iba1+ cells) were analyzed by immunohistochemistry in retinal wholemounts.
Results
Untreated db/db mice exhibited retinal thinning, RGC loss, and marked Iba1+ microglial activation. Both MiS1 and baricitinib treatments significantly attenuated retinal thinning, with MiS1 preserving retinal thickness at levels comparable to those of nondiabetic controls. RGC numbers were partially preserved in both treated groups, although the increase compared with untreated db/db mice reached statistical significance only in baricitinib-treated animals. Both treatments improved microglial morphology, mainly by increasing arbor area, whereas their effects on Iba1+ cell numbers were layer and parameter dependent.