Clinical, cosmetic and investigational dermatology
Concentration-Optimized Astragalus Polysaccharide-Loaded PLCL Electrospun Dressings for the Treatment of Diabetic Wounds
Par Wang M, Gao X, Zhao Y et 3 autres.
Contenu en anglais
Résumé
Purpose
This study aims to fabricate electrospun nanofiber membranes composed of poly(L-lactide-co-ε-caprolactone) (PLCL) incorporated with Astragalus polysaccharides (APS) (APS@PLCL), and to evaluate their efficacy in enhancing diabetic wound healing, as well as to determine the optimal concentration of drug loading.
Methods
APS was incorporated into the PLCL spinning solution through an HP-β-CD inclusion complexation approach to fabricate APS@PLCL membranes with theoretical drug loadings of 0%, 3%, 6%, and 9%. The membranes were characterized in terms of morphology, mechanical properties, drug loading capacity, and in vitro drug release profiles. Biocompatibility was assessed using the CCK-8 assay and live/dead staining. The wound healing efficacy was evaluated in a full-thickness skin defect model in diabetic mice (n = 6 per group, 24 mice in total). Collagen deposition, angiogenesis, and the expression of inflammatory factors were analyzed via histological staining, RT-qPCR, ELISA and Western blot.