Antibiotics
Increasing Fosfomycin Resistance in Klebsiella pneumoniae Isolates Causing Bloodstream Infections in Hospitalized Adults: Seven Years of Surveillance
Par Tüzemen NÜ, Saticioglu İB, Duman M et 6 autres.
Contenu en anglais
Résumé
Introduction
We investigated seven-year temporal trends in fosfomycin resistance among Klebsiella pneumoniae bloodstream isolates and characterized resistant isolates phenotypically and molecularly.
Methods
We included 198 non-duplicate bloodstream isolates collected from hospitalized adults during 2017-2023. Fosfomycin MICs were determined by agar dilution and evaluated using the 2023 EUCAST intravenous clinical breakpoint, FDA susceptibility test interpretive criteria (STIC), and current EUCAST (T)ECOFF. Temporal trends were assessed by logistic regression. Phenotypic evidence of FosA-like enzyme production was assessed by PPF disk testing, acquired fosA variants by PCR and Sanger sequencing, and two representative isolates by Oxford Nanopore WGS.