International journal of women's health
Single-Cell Transcriptomics Reveals Rora-Associated Aberrant Granulosa Cell Differentiation in Polycystic Ovary Syndrome
Par Ji P, Sun S, Xu X et 1 autres.
Contenu en anglais
Résumé
Introduction
Polycystic ovary syndrome (PCOS) is characterized by follicular developmental arrest, yet the molecular basis underlying impaired granulosa cell (GC) differentiation remains incompletely understood.
Methods
We constructed a single-cell transcriptomic atlas of ovaries from DHEA-induced PCOS-like mice and matched vehicle controls, and leveraged spatial transcriptomics as an orthogonal reference for major cell-type annotation, followed by in-depth profiling of GC heterogeneity. Pseudotime inference, co-expression networks, and regulon activity analysis were integrated, and findings were validated in independent human GC datasets. In silico Rora perturbation, cell-cell communication analysis, and drug repositioning were additionally performed.