Journal of dental sciences
Changes of RNA expression in aging periodontal ligament cells
Par Kim SK, Ye JR, Kim G et 2 autres.
Contenu en anglais
Résumé
Background/purpose
Aging is a major risk factor for periodontal disease and is associated with impaired tissue homeostasis and regenerative capacity. The periodontal ligament (PDL) plays a central role in periodontal integrity; however, the molecular mechanisms underlying PDL cell aging remain incompletely understood.
Materials and methods
In this study, we established an in vitro passage-dependent cellular aging model using human periodontal ligament fibroblasts serially passaged from early to late stages. Transcriptomic changes associated with aging were analyzed using RNA sequencing, followed by principal component analysis, hierarchical clustering, pathway enrichment, and protein-protein interaction network analyses.