Frontiers in microbiology
Antimicrobial activity of ginsenoside Rh2 against Enterococcus faecalis and its biofilm
Par Liu C, Wang Y, Zhou S et 2 autres.
Contenu en anglais
Résumé
Background
Enterococcus faecalis (E. faecalis) is frequently associated with persistent root canal infections and treatment failure, and effective strategies for its elimination remain limited. Plant-derived compounds may provide alternative antimicrobial strategies for endodontic treatment.
Methods
In the present study, we evaluated the antimicrobial activity of 20(S)-ginsenoside Rh2 (Rh2), a bioactive compound derived from ginseng, against planktonic and biofilm forms of E. faecalis. The effects of Rh2 on extracellular polysaccharide (EPS)-associated carbohydrate content and biofilm metabolic activity were evaluated using the phenol-sulfuric acid and MTT assays, respectively. Biofilm structural alterations were examined by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The cytotoxicity of Rh2 toward L929 cells was assessed using an MTT assay, with sodium hypochlorite (NaClO) serving as the reference antimicrobial agent.