Genomic characterization of a mobile-element-associated resistance island in trimethoprim-sulfamethoxazole-resistant Stenotrophomonas maltophilia isolates sharing the same PFGE genotype

Background

Stenotrophomonas maltophilia is an opportunistic nosocomial pathogen with intrinsic multidrug resistance and limited therapeutic options. This study integrated phenotypic susceptibility testing, targeted molecular screening, PFGE and short-read whole-genome sequencing (WGS) to characterize resistance determinants and their local genomic context in clinical-culture isolates.