Antijerawat Ekstrak Teripang (Holothuria nobilis) dari Perairan Malalayang Dua Kota Manado Terhadap Pertumbuhan Propionibacterium acnes

Antibiotic resistance has driven the search for natural antibacterial agents to treat acne vulgaris caused by Propionibacterium acnes. This study aimed to evaluate the anti-acne efficacy and secondary metabolite composition of sea cucumber (Holothuria nobilis) extract from Malalayang Dua Waters, Manado. Extraction was performed via the maceration method using 95% ethanol. Active compounds were identified through phytochemical screening, while antibacterial activity was tested using the paper disc agar diffusion method at concentrations of 50%, 75%, and 100%. Phytochemical screening revealed that the extract contained alkaloids, flavonoids, saponins, and tannins. The antibacterial assay yielded inhibition zones of 10.4 mm (50%), 11.5 mm (75%), and 12.2 mm (100%), respectively, all falling into the strong category. In conclusion, the ethanol extract of Holothuria nobilis exhibits strong potential as a natural antibacterial agent against Propionibacterium acnes through the synergistic mechanisms of its secondary metabolites.